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Kansas State Veterinary Diagnostic Laboratory

Selection, Collection, and Submission of Samples

Histopathology and Immunohistochemistry Submission Guidelines

Tissue samples should be submitted in 10% neutral buffered formalin (NBF) in non-breakable plastic containers.

The ratio of formalin: tissue should be at least 10 parts NBF to 1 part tissue. When in doubt, collect specimens from multiple organs, including brain. Specimens from each individual animal can be pooled in a single container of NBF provided the specimens are small enough to maintain a 10:1 formalin:tissue ratio. Additionally, unstained slides can be submitted for routine histology and immunohistochemistry.

For tumor panels, if the original biopsy is not performed at the Kansas State Veterinary Diagnostic Laboratory, please submit the block with the best representative sample of the tumor. If the block cannot be sent, please send 6 unstained slides and two tubes with 5 scrolls each from the block.  In addition, a copy of the previous biopsy report needs to accompany the sample.

We also have a cryostat and can handle frozen sections.  Our digital scanner can scan whole slides and archive them.  We also have software that can analyze scanned slides for annotations (such as measurements) and immunohistochemical staining.

If you have questions about which samples to submit or about a specific test conducted by this lab, please contact Client Services at (785) 532-5650, toll free at 1-866-512-5650 orclientcare@vet.k-state.edu for special testing submission and fees.

Following is a list of the antigens that we are able to detect by specific labeled antibody.

Immunohistochemical Markers/Antibodies