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Kansas State Veterinary Diagnostic Laboratory

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Tests Especially Valuable in the Spring

Bovine Abortion Tissue PCR Panel

KSVDL is now offering a panel that includes testing for five Leptospira serovars (L. canicola,

L.grippotyphosa, L. hardjo, L. icterohaemorrhagiae, L. pomona), IBR, BVD type 1 and 2, and Neospora caninum in fetal tissue. 

Specimens: 1 gm fresh tissues that include: liver, kidney, skeletal muscle, spleen, lung, placenta

Days tested: M-F

Estimated turnaround:  1-2 days

 

Bovine Abortion Serology Panel

This serology panel includes testing for Infectious Bovine Rhinotracheitis (IBR), Bovine Viral Diarrhea (BVD 1 and 2), Lepto (includes all five serovars), Neospora caninum, Brucella abortus

Specimens: 2-3 ml serum (red top) or preservative tube

Days tested: M-F

Estimated turnaround:  4-7 days

 

Bovine Neonatal Enteric Diarrhea PCR Panels

For all three panels either 5 gms of feces of 1 gm fresh intestine can be submitted in a sterile container.

This PCR is completed Monday through Friday and expected Turnaround Time is 1-2 days.

1. Bovine Neonatal Enteric Diarrhea PCR Panels

This PCR detects:
Viral pathogens:
Coronavirus Rotavirus (group A)
Bacterial & Protozoal pathogens
E. coli K99
Salmonella sp.
Cryptosporidia sp.

2. Bovine Neonatal Enteric Diarrhea PCR Viral Panel

This PCR detects:
Viral pathogens:
Coronavirus Rotavirus (group A)

3. Bovine Neonatal Enteric Diarrhea PCR Bacterial:Protozoal Panel

This PCR detects:
Bacterial and Protozoal pathogens:
E. coli K99
Salmonella sp.
Cryptosporidia sp.

 

For more information contact KSVDL Client Care at 866-512-5650 or
clientcare@vet.k-state.edu.

 

Canine and Feline Core Vaccine Titer Screening

Rabies Titer Test

The Micro Rabies Screen Test (MRS) is a serum neutralization assay based on the RFFIT. Since The MRS test measures rabies virus neutralizing antibody (RVNA) titers, it can be used as a rapid screening for evaluating the need for rabies boosters.

A review of rabies challenge-studies indicates that there is a positive correlation between rabies virus neutralizing antibody (RVNA) titers and the level of protection after virus challenge. Pre-exposure vaccination coupled with a RVNA titer at or above 0.5 IU/mL indicates greater assurance of protection than does current vaccination status.

Using the MRS test results:
If the RVNA value is <0.5 IU/mL, KSVDL recommends administering a rabies booster.
If the RVNA value is >0.5 IU/mL, KSVDL does not recommend a rabies booster, but to recheck in one year.

Other Core Vaccine Serologic Tests

The current trend is to carefully assess each patient’s disease risk to determine if vaccine(s) are necessary and if so, which vaccine(s) would be appropriate. The use of serological titers can be a valuable tool in making those vaccine decisions especially in animals with histories of a previous vaccine reaction; those individuals prone to allergic reactions; in those specific cases where concerns of “over vaccination” persist.

 

CANINE:  There is an excellent correlation between a “positive” titer and protection against viral challenge with canine distemper virus (CDV), canine adenovirus 1(CAV1), canine parvovirus2 (CPV), and Rabies (RV).

CDV: SN > 1: 32
CAV1:SN > 1: 32
CPV2:HI > 1: 80

 

FELINE: In cats there is an excellent correlation between a “positive” titer and protection against challenge with the feline panleukopenia virus (FPL) and rabies virus (RV) but only a good correlation with feline herpesvirus (FHV1) and feline calicivirus (FCV) protection.

FPL:HI > 1: 16
FCV: SN > 1:16
FHV1:SN > 1:16

 

For more information on these test options, please contact KSVDL Client Care at
clientcare@vet.k-state.edu or 866-884-3867

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